# Variant Report

<span style="font-size: 12pt; font-family: Montserrat, sans-serif; color: #000000; background-color: transparent; font-weight: 400; font-style: normal; font-variant: normal; white-space: pre-wrap;">Variant calling pipelines contains two exclusive sections.</span>

<span style="font-size: 14pt; font-family: Montserrat, sans-serif; color: #000000; background-color: transparent; font-weight: bold; font-style: normal; font-variant: normal; white-space: pre-wrap;"> </span><span style="font-size: 14pt; font-family: Montserrat, sans-serif; color: #6aa84f; background-color: transparent; font-weight: bold; font-style: normal; font-variant: normal; white-space: pre-wrap;">Mapping</span>

<span style="font-size: 12pt; font-family: Montserrat, sans-serif; color: #232a3c; background-color: transparent; font-weight: 400; font-style: normal; font-variant: normal; white-space: pre-wrap;">This section provides details of the abundance/mapping statistics. This section also has three sub-sections:This</span>

1. 1. 1. <span style="font-size: 12pt; font-family: Montserrat, sans-serif; background-color: transparent; font-weight: 400; font-style: normal; font-variant: normal; text-decoration-line: underline; text-decoration-skip-ink: none; white-space: pre-wrap;">Data Table</span><span style="font-size: 12pt; font-family: Montserrat, sans-serif; background-color: transparent; font-weight: 400; font-style: normal; font-variant: normal; white-space: pre-wrap;">: This (Fig. 1) shows several alignment statistics such as the number of total processed reads, the number of mapped or multi-mapped reads, and the uniquely mapped reads.</span>
        2. <span style="font-size: 12pt; font-family: Montserrat, sans-serif; background-color: transparent; font-weight: 400; font-style: normal; font-variant: normal; text-decoration-line: underline; text-decoration-skip-ink: none; white-space: pre-wrap;">Sample Coverage</span><span style="font-size: 12pt; font-family: Montserrat, sans-serif; background-color: transparent; font-weight: 400; font-style: normal; font-variant: normal; white-space: pre-wrap;">: Allows users to explore the alignment quality through a series of plots. </span><span style="font-size: 12pt; font-family: Montserrat, sans-serif; background-color: transparent; font-weight: 400; font-style: normal; font-variant: normal; white-space: pre-wrap;">Sample depth of coverage in total read counts. </span><span style="font-size: 12pt; font-family: Montserrat, sans-serif; background-color: transparent; font-weight: 400; font-style: normal; font-variant: normal; white-space: pre-wrap;">Sample depth of coverage in percentages. </span><span style="font-size: 12pt; font-family: Montserrat, sans-serif; background-color: transparent; font-weight: 400; font-style: normal; font-variant: normal; white-space: pre-wrap;">On-target mapping quality in a 96-well plate format</span>
        3. <span style="font-size: 12pt; font-family: Montserrat, sans-serif; background-color: transparent; font-weight: 400; font-style: normal; font-variant: normal; text-decoration-line: underline; text-decoration-skip-ink: none; white-space: pre-wrap;">Read Lengths:</span><span style="font-size: 12pt; font-family: Montserrat, sans-serif; background-color: transparent; font-weight: 400; font-style: normal; font-variant: normal; white-space: pre-wrap;"> Histograms and 96-well plate plots show the read length distributions for mapped and unmapped reads.</span>
        4. <span style="font-size: 12pt; font-family: Montserrat, sans-serif; background-color: transparent; font-weight: 400; font-style: normal; font-variant: normal; text-decoration-line: underline; text-decoration-skip-ink: none; white-space: pre-wrap;">Genome Browser:</span><span style="font-size: 12pt; font-family: Montserrat, sans-serif; background-color: transparent; font-weight: 400; font-style: normal; font-variant: normal; white-space: pre-wrap;"> Aligned reads against the reference genome can be viewed for each sample. The genome browser is interactive and allows exploratory analysis.</span>

![Fig. 1. Alignment statistics in the report.](https://docs.stanome.com/uploads/images/gallery/2022-01/embedded-image-ympnqso2.png)

<span style="font-size: 14pt; font-family: Montserrat, sans-serif; color: #000000; background-color: transparent; font-weight: bold; font-style: normal; font-variant: normal; white-space: pre-wrap;"> </span><span style="font-size: 14pt; font-family: Montserrat, sans-serif; color: #6aa84f; background-color: transparent; font-weight: bold; font-style: normal; font-variant: normal; white-space: pre-wrap;">Variant Calling</span>

<span style="font-size: 12pt; font-family: Montserrat, sans-serif; color: #232a3c; background-color: transparent; font-weight: 400; font-style: normal; font-variant: normal; white-space: pre-wrap;">This section provides details of the abundance/mapping statistics. This section also has three sub-sections: </span>

1. 1. 1. 1. <span style="font-size: 12pt; font-family: Montserrat, sans-serif; background-color: transparent; font-weight: 400; font-style: normal; font-variant: normal; text-decoration-line: underline; text-decoration-skip-ink: none; white-space: pre-wrap;">Call Rate Summary</span><span style="font-size: 12pt; font-family: Montserrat, sans-serif; background-color: transparent; font-weight: 400; font-style: normal; font-variant: normal; white-space: pre-wrap;">: Provides a summary of the genotype calls.</span>
            2. <span style="font-size: 12pt; font-family: Montserrat, sans-serif; background-color: transparent; font-weight: 400; font-style: normal; font-variant: normal; text-decoration-line: underline; text-decoration-skip-ink: none; white-space: pre-wrap;">Target Call Rates</span><span style="font-size: 12pt; font-family: Montserrat, sans-serif; background-color: transparent; font-weight: 400; font-style: normal; font-variant: normal; white-space: pre-wrap;">: Genotype calling metrics for the top 100 targets are shown in the table format. The complete list can be downloaded from the </span><span style="font-size: 12pt; font-family: Montserrat, sans-serif; background-color: transparent; font-weight: bold; font-style: normal; font-variant: normal; white-space: pre-wrap;">Reports</span><span style="font-size: 12pt; font-family: Montserrat, sans-serif; background-color: transparent; font-weight: 400; font-style: normal; font-variant: normal; white-space: pre-wrap;"> section. The position field is cross-linked to the Variant Browser. This section also shows:</span>
                1. <span style="font-size: 12pt; font-family: Montserrat, sans-serif; background-color: transparent; font-weight: 400; font-style: normal; font-variant: normal; white-space: pre-wrap;">Genotype call distribution</span>
                2. <span style="font-size: 12pt; font-family: Montserrat, sans-serif; background-color: transparent; font-weight: 400; font-style: normal; font-variant: normal; white-space: pre-wrap;">Genotype Heatmap</span>
            3. <span style="font-size: 12pt; font-family: Montserrat, sans-serif; background-color: transparent; font-weight: 400; font-style: normal; font-variant: normal; text-decoration-line: underline; text-decoration-skip-ink: none; white-space: pre-wrap;">Sample Call Rates</span><span style="font-size: 12pt; font-family: Montserrat, sans-serif; background-color: transparent; font-weight: 400; font-style: normal; font-variant: normal; white-space: pre-wrap;">: Histograms and 96-well plate plots show the call rate distributions from all the samples.</span>
            4. <span style="font-size: 12pt; font-family: Montserrat, sans-serif; background-color: transparent; font-weight: 400; font-style: normal; font-variant: normal; text-decoration-line: underline; text-decoration-skip-ink: none; white-space: pre-wrap;">Genotypes:</span><span style="font-size: 12pt; font-family: Montserrat, sans-serif; background-color: transparent; font-weight: 400; font-style: normal; font-variant: normal; white-space: pre-wrap;"> Shows table of genotypes obtained for each marker across all the samples</span>
            5. <span style="font-size: 12pt; font-family: Montserrat, sans-serif; background-color: transparent; font-weight: 400; font-style: normal; font-variant: normal; text-decoration-line: underline; text-decoration-skip-ink: none; white-space: pre-wrap;">Variant Browser:</span><span style="font-size: 12pt; font-family: Montserrat, sans-serif; background-color: transparent; font-weight: 400; font-style: normal; font-variant: normal; white-space: pre-wrap;"> Sequencing reads support for each variant can be viewed for each sample (Fig. 2). The genome browser is interactive and allows exploratory analysis.</span>

![Fig. 2. The variant browser in the report.](https://docs.stanome.com/uploads/images/gallery/2022-01/embedded-image-dcihgcll.png)